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The Entamoeba complex contains two morphologically identical species: Entamoeba dispar, which is avirulent, and Entamoeba histolytica, which may be an avirulent intestinal commensal or lead to serious disease Disease follows penetration of the intestinal wall, resulting in diarrhea, dysentery, and extraintestinal disease, most commonly liver abscess E histolytica infections are present worldwide but are most prevalent in subtropical and tropical areas under conditions of crowding, poor sanitation, and poor nutrition Of the estimated 500 million persons worldwide infected with Entamoeba, most are infected with E dispar and an estimated 10% with E histolytica Mortality from invasive E histolytica infections is estimated at 100,000 per year Humans are the only established E histolytica host Transmission occurs through ingestion of cysts from fecally contaminated food or water, facilitated by personto-person spread, flies and other arthropods as mechanical vectors, and use of human excrement as fertilizer Urban outbreaks have occurred because of common-source water contamination

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Diagnosis is by finding E histolytica or its antigen or by serologic tests However, each method has limitations 1 Intestinal amebiasis Diagnosis is most commonly made by identifying organisms in the stool E histolytica and E dispar cannot be distinguished, but the identification of amebic trophozoites or cysts in a symptomatic patient is highly suggestive of amebiasis Stool evaluation for organisms is not highly sensitive (~30 50% for amebic colitis), and at least three stool

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specimens should be evaluated after concentration and staining Multiple serologic assays are available; these tests are fairly sensitive, although sensitivity is lower (~70% in colitis) early in illness, and they cannot distinguish recent and old disease, as they remain positive for years after infection A commercially available stool antigen test (TechLab) offers improved sensitivity (> 90% for colitis); it requires fresh or frozen (not preserved) stool specimens Colonoscopy of uncleansed bowel typically shows no specific findings in mild intestinal disease; in severe disease, ulcers may be found with intact intervening friable mucosa, resembling inflammatory bowel disease Examination of fresh ulcer exudate for motile trophozoites and for E histolytica antigen may yield a diagnosis 2 Hepatic abscess Abscesses can be identified by ultrasonography, CT, or MRI, with round or oval low-density nonhomogeneous lesions, with abrupt transition from normal liver to the lesion, and hypoechoic centers Abscesses are most commonly single, but more than one may be present The right lobe is usually involved Serologic tests for antiamebic antibodies are almost always positive, except very early in the infection Thus, a negative test in a suspicious case should be repeated in about a week The stool E histolytica antigen test is positive in ~40% of cases; a serum antigen test

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is under development Examination of stools for the organisms or antigen is frequently negative As imaging studies cannot distinguish amebic and pyogenic abscesses, when a diagnosis is not available from serologic studies, percutaneous aspiration may be indicated, ideally with an image-guided needle Aspiration typically yields brown or yellow fluid Detection of organisms in the aspirate is uncommon, but detection of E histolytica antigen is very sensitive and diagnostic The key risk of aspiration is peritoneal spillage leading to peritonitis from amebas or other (pyogenic or echinococcal) organisms

Treatment of amebiasis generally entails the use of metronidazole or tinidazole to eradicate tissue trophozoites and a luminal amebicide to eradicate intestinal cysts (Table 35 6) Asymptomatic infection with E dispar does not require therapy This organism cannot be differentiated morphologically from E histolytica, but with negative serology E dispar colonization is likely, and treatment is not indicated Colonization with E histolytica is generally treated with a luminal agent Effective luminal agents are diloxanide furoate (500 mg three times daily with meals for 10 days), iodoquinol (diiodohy-

Clinical Setting Asymptomatic intestinal infection Drugs of Choice and Adult Dosage Luminal agent: Diloxanide furoate, 500 mg orally three times daily for 10 days or Iodoquinol, 650 mg orally three times daily for 21 days or Paromomycin, 10 mg/kg orally three times daily for 7 days Mild to moderate intestinal infection Metronidazole, 750 mg orally three times daily (or 500 mg IV every 6 hours) for 10 days or Tinidazole, 2 g orally daily for 3 days plus Luminal agent (see above) Severe intestinal infection Metronidazole, 750 mg orally three times daily (or 500 mg IV every 6 hours) for 10 days or Tinidazole, 2 g orally daily for 3 days plus Luminal agent (see above) Hepatic abscess, ameboma, and other extraintestinal disease Metronidazole, 750 mg orally three times daily (or 500 mg IV every 6 hours) for 10 days or Tinidazole, 2 g orally daily for 3 days plus Luminal agent (see above)

1 2 2

Specific information regarding common presentations of endocrine disorders appears on CMDT Online at http:// wwwaccessmedicinecom

Umesh Masharani, MB, BS, MRCP(UK)

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