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The rest of the code deals with the physical layout of the application area, especially removing the borders. If you want to see the CSS listing in its entirety, flip to Appendix A and check out Listing A-2. Thanks for the basis for this style sheet must go to Mihai Parparita, who released the original underneath the Creative Commons Attribution-ShareAlike license at http://persistent.info/archives/2004/10/05/gmail-skinning. Now that you have your new style sheet applied, you can get down to the business of ridding Gmail of advertising.

, Sieminski, AL, Bursac, PM, Vunjak-Novakovic, G, Freed, LE (2001a) IGF-I and mechanical environment interact to modulate engineered cartilage development Biochem Biophys Res Commun 286: 909 915 Gooch, KJ, Kwon, JH, Blunk, T, Langer, R, Freed, LE, Vunjak-Novakovic, G (2001b) Effects of mixing intensity on tissue-engineered cartilage Biotechnol Bioeng 72: 402 407 Grande, DA, Halberstadt, C, Naughton, G, Schwartz, R, Manji, R (1997) Evaluation of matrix scaffolds for tissue engineering of articular cartilage grafts J Biomed Mater Res 34: 211 220 Hauselmann, HJ, Fernandes, RJ, Mok, SS, Schmid, TM, Block, JA, Aydelotte, MB, Kuettner, KE, Thonar, EJM (1994) Phenotypic stability of bovine articular chondrocytes after long-term culture in alginate beads J Cell Sci.

Protocol (a) Collect beads in a sterile 50-ml tube with a sterile spatula as follows: i) Bovine, collect 300 beads/insert (See Protocol 7.6) ii) Human, collect 350 beads/insert iii) Porcine, collect 300 350 beads/insert (b) Add 20 ml alginate dissolving buffer to each tube. (c) Mix the tube gently. Do Not Vortex. Let it sit at room temperature for 30 min. (d) After the beads have dissolved, centrifuge at 110 g for 10 min at 4 C. (e) Remove the supernate and wash the cell pellet 2 with saline wash. Centrifuge at 110 g for 10 min at 4 C each time, removing the wash solutions with a Pasteur pipette connected to the vacuum line in the hood. Leave approximately 2 ml after each aspiration. (f) Wash the cell pellet once with 20 ml isolation medium. (g) Centrifuge at 110 g for 10 min at 4 C. (h) Resuspend in culture medium as required.

01, 10, and 11. This is a 2-bit code. A 3-bit code would yield eight different binary numbers. We nd, then, that the number of total possible different binary combinations given a code of n binary symbols (bits) is 2n . A 7-bit code has 128 different binary combinations (i.e., 27 128). For the quantization process, we want to present to the coder a discrete voltage value. Suppose our quantization steps were on 0.1-V increments and our voltage measure for one sample was 0.37 V. That would have to be rounded off to 0.4 V, the nearest discrete value. Note here that there is a 0.03-V error, the difference between 0.37 V and 0.40 V. Figure 6.3 shows one cycle of the PAM wave appearing in Figure 6.1, where we use a 4-bit code. In the gure a 4-bit code is used that allows 16 different binary-coded possibilities or levels between +1 V and 1 V. Thus we can assign eight possibilities above the origin and eight possibilities below the origin. These 16 quantum steps are coded as follows:

107: 17 27 Haynesworth, SE, Reuben, D, Caplan, AI (1998) Cell-based tissue engineering therapies: The in uence of whole body physiology Adv Drug Deliv Res 33: 3 14 Heath, CA (2000) The effects of physical forces on cartilage tissue engineering Biotechnol Genet Eng Rev 17: 533 551 Hoemann, CD, Sun, J, Legare, A, McKee, MD, Ranger, P, Buschmann, MD (2001) A thermosensitive polysaccharide gel for cell delivery in cartilage repair Trans Orthop Res Soc 26 Hollander, AP, Heath eld, TF, Webber, C, Iwata, Y, Bourne, R, Rorabeck, C, Poole, RA (1994) Increased damage to type II collagen in osteoarthritic articular cartilage detected by a new immunoassay J Clin Invest 93: 1722 1732 Johnstone, B, Hering, TM, Caplan, AI, Goldberg, VM.