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in which k is known as the Boltzmann constant. The form of Equations (1.7) and (1.8) gives some insights into the effects of temperature T on V . The higher is T, the smaller is C and hence the higher is the value of average potential energy V . In other words, increasing T increases system average potential energy V by disorganising the given system, thereby minimising mutual dipole orientation and attractive interaction effects, which otherwise act to minimise V and stabilise the system. Electrostatic interactions are involved in stabilising the structures of biological macromolecules. Globular protein surfaces are covered with charged amino-acid residues that interact with each other to form surface stabilising salt-links. Moreover, surface clusters of charges approximate to arrays of monopoles, dipoles and quadrupoles, hence all the electrostatic equations apply. These same surface clusters of monopoles, dipoles and quadrupoles also radiate electrostatic force-lines into solution, with consequences for protein ligand interactions and molecular recognition phenomena (see 7). In the case of nucleic acids, charge charge repulsions between the anti-parallel phosphodiester chains could be suf cient to cause chain separation. However, phosphate negative charges are usually counterbalanced by close neighbour counter-ions (cations or cationic proteins) that modulate the magnitude of the negative charges. In this case, the charges then provide suf cient charge charge repulsion to maintain the positions of the anti-parallel phosphodiester chains, preventing hydrophobic collapse, without inappropriately perturbing the structure overall.

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Holt, SC and ER Leadbetter 1969 Comparative ultrastucture of selected aeorobic spore-forming bacteria: a freeze-etching study Bacteriol Rev 33:346 378 Horowitz, A, MI Krichevsky, and RM Atlas 1983 Characteristics and diversity of subarctic marine oligotrophic, stenoheterotrophic, and euryheterotrophic bacterial populations Can J Microbiol 29:527 535 Jannasch, HW 1969 Estimation of bacterial growth rates in natural waters J Bacteriol 99:156 160 Jannasch, HW 1979 Microbial ecology of aquatic low nutrient habitats In: M Shilo (ed) Extreme Environments, pp 243 260 Dahlem Konferenzen Life Sciences Research Report No 13 Verlag Chemie, Weinheim Kelly, DP and AP Wood 2006 The chemolithotrophic prokaryotes In: MW Dworkin, S Falkow, E Rosenberg, K-H Schleifer, and E Stackebrandt (eds) The Prokaryotes, Vol 2, 3rd edn, pp 441 456 Springer-Verlag, New York Kluyver, AJ and CB van Niel 1954.

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The Microbe s Contributions to Biology Harvard University Press Cambridge, MA Koch, AL 1971 The adaptive response of Escherichia coli to feast and famine existence Adv Microbial Physiol 6:147 217 Koch, AL 1997 Microbial physiology and ecology of slow growth Microbiol Molec Biol Rev 61:305 318 Kolker, E, AF Picone, MY Galperin, et al 2005 Global pro ling of Shewanella oneidensis MR-1: expression of hypothetical genes and improved functional annotations Proc Natl Acad Sci USA 102:2099 2104 Kolter, R, A Siegele, and A Tormo 1993 The stationary phase of the bacterial life cycle Annu Rev Microbiol 47:855 874 Konstantinidis, KT and JM Tiedje 2004a Microbial diversity and genomics In: J Zhou, DK Thompson, JM Tiedje, and Y Xu (eds) Microbial Functional Genomics, pp 21 40 Wiley and Sons, New York Konstantinidis, KT and JM Tiedje 2004b.

CHAPTER 18 Edge-Lit Holography light -the likely choice for hologram recording -the amplitude of the reflected wave is given by:

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To save space on your Mac s hard drive, you ll usually want to trim down your clips by selecting only the footage you want to use. Use these techniques to select the footage: To select a standard-length chunk of video, click the clip. iMovie displays a yellow outline around it, starting from the point where you clicked. By default, iMovie selects four seconds.

Trends between gene content and genome size in prokaryotic species with larger genomes Proc Natl Acad Sci USA 101:3160 3165 Lengeler, JW, G Drews, and HG Schlegel (eds) 1999 Biology of Prokaryotes Blackwell Science, Stuttgart..

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lash 8 provides a WebService class written in ActionScript (that is, it s not native to Flash Player) that lets you work with SOAP Web services from Flash. The WebService class is included with both Flash 8 Basic and Flash 8 Professional. Flash 8 Professional also has a set of data components including a WebServiceConnector component that allows you to connect to Web services from your Flash applications. Although the WebServiceConnector component may seem simpler initially, it is not as flexible as the WebService class, and it is more difficult to build into most applications. The WebServiceConnector component uses the WebService class as the underlying engine, so there is nothing in terms of basic Web service functionality that the WebServiceConnector component can do that the WebService class cannot. Therefore, in this chapter we ll look at how to work with the lower-level WebService class directly.

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